Overview

Dr Bertram Daum

Research Fellow at the Living Systems Institute

College of Mathematics, Engineering and Physical Science

Telephone: 01392727455

Room: T03.17, Living Systems Institute

Follow me on Twitter: @DaumLaboratory

Research

Archaea are ubiquitous microorganisms that are the ancestors of all eukaryotic life and inhabit diverse environments ranging from the most extreme to the human body. Archaea that are part of the human microbiome are increasingly recognised as key health factors in metabolic conditions such as obesity. Moreover, protein complexes derived from extremophilic archaea are ultra-stable and highly resilient to extreme conditions such as heat, pH and salt and are thus of great interest for bio- and nanotechnology. My lab employs state-of-the-art single particle electron cryo-microscopy and cryo-tomography to investigate the structure and function of archaeal surface proteins. These include S-layers, which form highly stable cage-like proteinaceous cell walls and the archaellum, a filamentous molecular machine used for rotary propulsion, surface adherence, biofilm formation and cell-cell communication. Our work will provide detailed information about how archaea move through and interact with their (microbiomal) environment and inform new approaches to exploit s-layers and the archaellum machinery in nanotechnology and drug delivery.

Group Members: 

Dr Kelly Sanders (Lab Technician)

Dr Mathew McLaren (Experimental Officer for cryoEM)

Dr Lavinia Gambelli (Postdoc) 

In the news: 

http://www.exeter.ac.uk/news/featurednews/title_603910_en.html

https://phys.org/news/2017-08-scientists-snapshots-molecular-propeller-degrees.html

http://www.sciencenewsline.com/news/2017082417090076.html

http://www.hitechdays.com/browser/253317/

Equipment

Our brand-new lab is home to cultivation equipment for thermophiles, a cryoEM sample preparation facility, a cryo-capable 120 kV FEI T12 electron microscope for sample screening and a cutting-edge GPU computer cluster for image processing. For high resolution imaging, we share a 200 kV Talos Arctica electron microscope with the University of Bristol.

Qualifications

2014: Dr phil. nat. in Biology, University of Frankfurt, Germany

2008:  MsSc in Biology, University of Kassel, Germany

Career

2017 – present: Research Fellow at the Living Systems Institute, University of Exeter, UK

2014 – 2017: Postdoctoral researcher at the Max-Planck-Institute of Biophysics, Frankfurt, Germany   

2008 – 2014: PhD research at the Max-Planck-Institute of Biophysics, Frankfurt, Germany   

Awards

2014: Otto-Hahn Medal of the Max Planck Society

2014: Young Investigator’s Award of the Bethge Foundation for Electron Microscopy

Publications

Daum B, Gold V. (2018) Twitch or swim: towards the understanding of prokaryotic motion based on the type IV pilus blueprint. Biol Chem. 2018 Jun 27;399(7):799-808.

Quax TEF, Daum B (2018) Structure and assembly mechanism of virus-associated pyramids. Biophys Rev. 2018 Apr;10(2):551-557. doi: 10.1007/s12551-017-0357-4.

Daum B, Vonck J, Bellack A, Chaudhury P, Reichelt R, Albers SV, Rachel R, Kühlbrandt W (2017) Structure and in situ organisation of the Pyrococcus furiosus archaellum machinery. Elife, Jun 27;6. pii: e27470.

Daum B, Auerswald A, Gruber T, Hause G, Balbach J, Kühlbrandt W†, Meister A (2016)  Supramolecular organization of the human N-BAR domain and its role in shaping the sarcolemma membrane. Journal of Structural Biology, 194(3):375-82.

Perras A, Daum B, Ziegler C, Takahashi LK, Ahmed M, Wanner G, Klingl G, Leitinger G, Kolb-Lenz D, Gribaldo S, Auerbach A, Mora M, Probst AJ, Bellack A and Moissl-Eichinger C (2015) S-layers at a second glance? Altiarchaeal grappling hooks (hami) resemble archaeal S-layer proteins in structure and sequence. Frontiers in Microbiology 6:543

Davies KM, Daum B, Gold V, Mühleip AW, Brandt T, Blum TB, Mills DJ, Kühlbrandt W (2014) Visualization of  ATP-synthase dimers in mitochondria by electron cryotomography. Journal of Visualized Experiments, (91):51228

Daum B, Quax TEF, Sachse M, Mills D, Reimann J, Yildiz Ö, Häder S, Saveanu C, Forterre P, Albers SV, Kühlbrandt W and Prangishvili D (2014) Self-assembly of the universal membrane-remodeling protein PVAP into 7-fold virus-associated pyramids, Proceedings of the National Academy of Sciences of the United States of America, 111(10):3829-34

Quemin ERJ, Lucas S, Daum B, Quax EFT, Kühlbrandt W, Forterre P, Albers SV, Prangishvili D, Krupovic M. (2013) First insights into the entry process of hyperthermophilic archaeal viruses, Journal of Virology 87(24):13379-85.

Daum B, Walter A, Horst A, Osiewacz H, Kühlbrandt W (2013) Age-dependent dissociation of ATP synthase dimers and loss of inner membrane cristae in mitochondria. Proceedings of the National Academy of Sciences of the United States of America 111:15301-10

Davies KM & Daum B (2013) Role of cryo-ET in membrane bioenergetics research. Biochemical Society Transactions 41(5):1227-34.

Sommer MS, Daum B, Gross LE, Weis BL, Mirus O, Abram L, Maier UG, Kühlbrandt W, Schleiff E (2011) Chloroplast Omp85 proteins change orientation during evolution. Proceedings of the National Academy of Sciences of the United States of America 108: 13841-13846

Davies KM, Strauss M, Daum B*, Kief JH, Osiewacz HD, Rycovska A, Zickermann V, Kühlbrandt W (2011) Macromolecular organization of ATP synthase and complex I in whole mitochondria. Proceedings of the National Academy of Sciences of the United States of America 108: 14121-14126

Daum B & Kühlbrandt W (2011) Electron tomography of plant thylakoid membranes. Journal of Experimental Botany 62: 2393-2402

Brust D, Daum B, Breunig C, Hamann A, Kühlbrandt W, Osiewacz HD (2010) Cyclophilin D links programmed cell death and organismal aging in Podospora anserina. Aging Cell 9: 761-775

Daum B, Nicastro D, Austin J, 2nd, McIntosh JR, Kühlbrandt W (2010) Arrangement of photosystem II and ATP synthase in chloroplast membranes of spinach and pea. The Plant Cell 22: 1299-1312 

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Publications

_{Copyright Notice: Any articles made available for download are for personal use only. Any other use requires prior permission of the author and the copyright holder.}

| 2024 | 2023 | 2022 | 2021 | 2020 | 2019 | 2018 | 2017 | 2016 | 2015 | 2014 | 2013 | 2012 | 2011 | 2010 |

2024

• Gambelli L, McLaren M, Conners R, Sanders K, Gaines MC, Clark L, Gold VAM, Kattnig D, Sikora M, Hanus C. (2024) Structure of the two-component S-layer of the archaeon Sulfolobus acidocaldarius, eLife, volume 13, DOI:10.7554/elife.84617. [PDF]
• Kuiper BP, Schöntag AMC, Oksanen HM, Daum B, Quax TEF. (2024) Archaeal virus entry and egress, microLife, volume 5, DOI:10.1093/femsml/uqad048.
• Buzzard E, McLaren M, Bragoszewski P, Brancaccio A, Ford H, Daum B, Kuwabara P, Collinson I, Gold V. (2024) The consequence of ATP synthase dimer angle on mitochondrial morphology studied by cryo-electron tomography, Biochem J, volume 481, no. 3, pages 161-175, DOI:10.1042/BCJ20230450. [PDF]

2023

• McLaren M, Conners R, Isupov MN, Gil-Diez P, Gambelli L, Gold VAM, Walter A, Connell SR, Williams B, Daum B. (2023) CryoEM reveals that ribosomes in microsporidian spores are locked in a dimeric hibernating state, NATURE MICROBIOLOGY, volume 8, no. 10, pages 1834-+, DOI:10.1038/s41564-023-01469-w. [PDF]
• McLaren M, Conners R, Isupov MN, Gil-Díez P, Gambelli L, Gold VAM, Walter A, Connell SR, Williams B, Daum B. (2023) CryoEM reveals that ribosomes in microsporidian spores are locked in a dimeric hibernating state, Nature Microbiology, volume 8, no. 10, pages 1834-1845, DOI:10.1038/s41564-023-01469-w. [PDF]
• Gaines MC, Sivabalasarma S, Isupov MN, Haque RU, McLaren M, Hanus C, Gold VAM, Albers S-V, Daum B. (2023) CryoEM reveals the structure of an archaeal pilus involved in twitching motility, DOI:10.1101/2023.08.07.552258. [PDF]
• Daum B, Gaines M, Sivabalasarma S, Isupov M, Haque R, McLaren M, Hanus C, Gold V, Albers S-V. (2023) CryoEM reveals the structure of an archaeal pilus involved in twitching motility, DOI:10.21203/rs.3.rs-3245295/v1. [PDF]
• Conners R, León-Quezada RI, McLaren M, Bennett NJ, Daum B, Rakonjac J, Gold VAM. (2023) Cryo-electron microscopy of the f1 filamentous phage reveals insights into viral infection and assembly, Nature Communications, volume 14, no. 1, article no. 2724, DOI:10.1038/s41467-023-37915-w. [PDF]
• Buzzard E, McLaren M, Bragoszewski P, Brancaccio A, Ford H, Daum B, Kuwabara P, Collinson I, Gold VAM. (2023) The consequence of ATP synthase dimer angle on mitochondrial morphology studied by cryo-electron tomography, DOI:10.1101/2023.02.02.526626.
• Troman LA, Alvira S, Daum B, Gold VAM, Collinson I. (2023) Interaction of the periplasmic chaperone SurA with the inner membrane protein secretion (SEC) machinery, Biochemical Journal, volume 480, no. 4, pages 283-296, DOI:10.1042/bcj20220480. [PDF]

2022

• Gaines MC, Isupov MN, Sivabalasarma S, Haque RU, McLaren M, Mollat CL, Tripp P, Neuhaus A, Gold VAM, Albers S-V. (2022) Electron cryo-microscopy reveals the structure of the archaeal thread filament, Nature Communications, volume 13, no. 1, article no. 7411, DOI:10.1038/s41467-022-34652-4. [PDF]
• Al-Otaibi N, Baatsen P, Bhella D, Brescia R, Bullough P, Daum B, de Bruin R, Frank R, Kühlbrandt W, López-Iglesias C. (2022) Tomographic analysis, CLEM: general discussion, Faraday Discussions, volume 240, no. 0, pages 142-151, DOI:10.1039/d2fd90060b.
• Conners R, León-Quezada RI, McLaren M, Bennett NJ, Daum B, Rakonjac J, Gold VAM. (2022) Cryo-electron microscopy of the f1 filamentous phage reveals a new paradigm in viral infection and assembly, DOI:10.1101/2022.11.03.514279. [PDF]
• Al-Otaibi N, Aminian J A, Anane RF, Baatsen P, Bakker SE, Bergeron J, Bharadwaj A, Bhella D, Braun T, Brescia R. (2022) Sample preparation in single particle cryo-EM: general discussion, Faraday Discuss, volume 240, no. 0, pages 81-100, DOI:10.1039/d2fd90059a. [PDF]
• Bakker SE, Bhella D, Brescia R, Bullough P, Clare DK, Daum B, Frank RAW, Gold VAM, Jackson Hirst I, Kühlbrandt W. (2022) Pushing the limits in single particle cryo-EM: general discussion, Faraday Discussions, volume 240, pages 312-322, DOI:10.1039/d2fd90063g. [PDF]
• Gambelli L, McLaren M, Conners R, Sanders K, Gaines MC, Clark L, Gold V, Kattnig D, Sikora M, Hanus C. (2022) Structure of the two-component S-layer of the archaeon Sulfolobus acidocaldarius, DOI:10.1101/2022.10.07.511299. [PDF]
• Troman L, Alvira S, Daum B, Gold VAM, Collinson I. (2022) INTERACTION OF THE PERIPLASMIC CHAPERONE SURA WITH THE INNER MEMBRANE PROTEIN SECRETION (SEC) MACHINERY, DOI:10.1101/2022.09.14.507990. [PDF]
• Gambelli L, Isupov MN, Daum B. (2022) Escaping the symmetry trap in helical reconstruction, Faraday Discussions, volume 240, pages 303-311, DOI:10.1039/d2fd00051b. [PDF]
• Gaines MC, Isupov MN, Sivabalasarma S, Haque RU, McLaren M, Tripp P, Neuhaus A, Gold V, Albers S-V, Daum B. (2022) Donor strand complementation, isopeptide bonds and glycosylation reinforce highly resilient archaeal thread filaments, DOI:10.1101/2022.04.26.489512.
• McLaren M, Gil-Diez P, Isupov M, Conners R, Gambelli L, Gold V, Walter A, Connell S, Williams B, Daum B. (2022) In situ structure of a dimeric hibernating ribosome from a eukaryotic intracellular pathogen, DOI:10.1101/2022.04.29.490036.
• Nuno de Sousa Machado J, Albers S-V, Daum B. (2022) Towards Elucidating the Rotary Mechanism of the Archaellum Machinery, Front Microbiol, volume 13, DOI:10.3389/fmicb.2022.848597. [PDF]
• Gambelli L, Isupov MN, Conners R, McLaren M, Bellack A, Gold V, Rachel R, Daum B. (2022) An archaellum filament composed of two alternating subunits, Nature Communications, volume 13, no. 1, article no. 710, DOI:10.1038/s41467-022-28337-1. [PDF]

2021

• Gambelli L, Mesman R, Versantvoort W, Diebolder CA, Engel A, Evers W, Jetten MSM, Pabst M, Daum B, van Niftrik L. (2021) The Polygonal Cell Shape and Surface Protein Layer of Anaerobic Methane-Oxidizing Methylomirabilislanthanidiphila Bacteria, Frontiers in Microbiology, volume 12, DOI:10.3389/fmicb.2021.766527. [PDF]
• Conners R, McLaren M, Łapińska U, Sanders K, Stone MRL, Blaskovich MAT, Pagliara S, Daum B, Rakonjac J, Gold VAM. (2021) CryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage, Nature Communications, volume 12, no. 1, article no. 6316, DOI:10.1038/s41467-021-26610-3. [PDF]
• Gambelli L, Isupov MN, Conners R, McLaren M, Bellack A, Gold V, Rachel R, Daum B. (2021) New insights into the architecture and dynamics of archaella, DOI:10.1101/2021.10.01.462426. [PDF]
• Conners R, McLaren M, Łapińska U, Sanders K, Stone MRL, Blaskovich MAT, Pagliara S, Daum B, Rakonjac J, Gold VAM. (2021) CryoEM structure of the outer membrane secretin channel pIV from the f1 filamentous bacteriophage, DOI:10.1101/2021.07.20.453082. [PDF]

2020

• Alvira S, Watkins DW, Troman LA, Allen WJ, Lorriman JS, Degliesposti G, Cohen EJ, Beeby M, Daum B, Gold VAM. (2020) Inter-membrane association of the Sec and BAM translocons for bacterial outer-membrane biogenesis, eLife, volume 9, DOI:10.7554/elife.60669. [PDF]
• Neuhaus A, Selvaraj M, Salzer R, Langer JD, Kruse K, Kirchner L, Sanders K, Daum B, Averhoff B, Gold VAM. (2020) Cryo-electron microscopy reveals two distinct type IV pili assembled by the same bacterium, Nature Communications, volume 11, no. 1, DOI:10.1038/s41467-020-15650-w.
• Gambelli L, Meyer BH, McLaren M, Sanders K, Quax TEF, Gold VAM, Albers SV, Daum B. (2020) Architecture and modular assembly of Sulfolobus S-layers revealed by electron cryotomography, Proceedings of the National Academy of Sciences of the United States of America, volume 116, no. 50, pages 25278-25286, DOI:10.1073/pnas.1911262116.

2019

• Neuhaus A, Selvaraj M, Salzer R, Langer JD, Kruse K, Sanders K, Daum B, Averhoff B, Gold VAM. (2019) A new twist on bacterial motility – two distinct type IV pili revealed by cryoEM, DOI:10.1101/720938. [PDF]
• Gambelli L, Meyer B, McLaren M, Sanders K, Quax TEF, Gold V, Albers S-V, Daum B. (2019) Architecture and modular assembly ofSulfolobusS-layers revealed by electron cryo-tomography, DOI:10.1101/677591. [PDF]
• Alvira S, Watkins DW, Troman L, Allen WJ, Lorriman J, Degliesposti G, Cohen EJ, Beeby M, Daum B, Gold VAM. (2019) Inter-membrane association of the Sec and BAM translocons for bacterial outer-membrane biogenesis, DOI:10.1101/589077. [PDF]

2018

• Daum B, Gold V. (2018) Twitch or swim: Towards the understanding of prokaryotic motion based on the type IV pilus blueprint, Biological Chemistry, volume 399, no. 7, pages 799-808, DOI:10.1515/hsz-2018-0157.
• Quax TEF, Daum B. (2018) Structure and assembly mechanism of virus-associated pyramids, Biophys Rev, volume 10, no. 2, pages 551-557, DOI:10.1007/s12551-017-0357-4. [PDF]

2017

• Davies K, Muehleip A, Blum T, Daum B, Anselmi C, Faraldo-Gómez J, Kühlbrandt W. (2017) Structure and in Situ Organization of ATP Sythase and Respiratory Chain Complexes, Biophysical Journal, volume 112, no. 3, pages 179a-180a, DOI:10.1016/j.bpj.2016.11.995.
• Daum B, Vonck J, Bellack A, Chaudhury P, Reichelt R, Albers SV, Rachel R, Kühlbrandt W. (2017) Structure and in situ organisation of the pyrococcus furiosus archaellum machinery, eLife, volume 6, DOI:10.7554/eLife.27470.

2016

• Daum B, Auerswald A, Gruber T, Hause G, Balbach J, Kühlbrandt W, Meister A. (2016) Supramolecular organization of the human N-BAR domain in shaping the sarcolemma membrane, Journal of Structural Biology, volume 194, no. 3, pages 375-382, DOI:10.1016/j.jsb.2016.03.017. [PDF]

2015

• Perras AK, Daum B, Ziegler C, Takahashi LK, Ahmed M, Wanner G, Klingl A, Leitinger G, Kolb-Lenz D, Gribaldo S. (2015) S-layers at second glance? Altiarchaeal grappling hooks (hami) resemble archaeal S-layer proteins in structure and sequence, Frontiers in Microbiology, volume 6, DOI:10.3389/fmicb.2015.00543. [PDF]

2014

• Davies KM, Daum B, Gold VAM, Mühleip AW, Brandt T, Blum TB, Mills DJ, Kühlbrandt W. (2014) Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography, Journal of Visualized Experiments, no. 91, DOI:10.3791/51228-v.
• Daum B, Quax TEF, Sachse M, Mills DJ, Reimann J, Yildiz Ö, Häder S, Saveanu C, Forterre P, Albers S-V. (2014) Self-assembly of the general membrane-remodeling protein PVAP into sevenfold virus-associated pyramids, Proceedings of the National Academy of Sciences, volume 111, no. 10, pages 3829-3834, DOI:10.1073/pnas.1319245111. [PDF]
• Davies KM, Daum B, Gold VAM, Mühleip AW, Brandt T, Blum TB, Mills DJ, Kühlbrandt W. (2014) Visualization of ATP synthase dimers in mitochondria by electron cryo-tomography, J Vis Exp, no. 91, DOI:10.3791/51228. [PDF]

2013

• Daum B, Walter A, Horst A, Osiewacz HD, Kühlbrandt W. (2013) Age-dependent dissociation of ATP synthase dimers and loss of inner-membrane cristae in mitochondria, Proceedings of the National Academy of Sciences, volume 110, no. 38, pages 15301-15306, DOI:10.1073/pnas.1305462110. [PDF]
• Davies KM, Daum B. (2013) Role of cryo-ET in membrane bioenergetics research, Biochemical Society Transactions, volume 41, no. 5, pages 1227-1234, DOI:10.1042/bst20130029. [PDF]
• Quemin ERJ, Lucas S, Daum B, Quax TEF, Kühlbrandt W, Forterre P, Albers S-V, Prangishvili D, Krupovic M. (2013) First Insights into the Entry Process of Hyperthermophilic Archaeal Viruses, Journal of Virology, volume 87, no. 24, pages 13379-13385, DOI:10.1128/jvi.02742-13. [PDF]

2012

• Davies KM, Anselmi C, Daum B, Faraldo-Gómez JD, Kühlbrandt W. (2012) In situ structure of the mitochondrial F1Fo ATP synthase dimer and its role in shaping membrane morphology, Biochimica et Biophysica Acta (BBA) - Bioenergetics, volume 1817, pages s11-s12, DOI:10.1016/j.bbabio.2012.06.041.
• Davies KM, Daum B, Kühlbrandt W, Anselmi C, Faraldo-Gómez J. (2012) Structure Of The Mitochondrial ATP Synthase And Its Role In Shaping Mitochondria Cristae, Microscopy and Microanalysis, volume 18, no. S2, pages 56-57, DOI:10.1017/s1431927612002139.
• Daum B, Walter A, Horst A, Heide H, Werner A, Brandt U, Osiewacz HD, Kühlbrandt W. (2012) Macromolecular reorganization of mitochondria during aging, Biochimica et Biophysica Acta (BBA) - Bioenergetics, volume 1817, DOI:10.1016/j.bbabio.2012.06.264.

2011

• Daum B, Kuhlbrandt W. (2011) Electron tomography of plant thylakoid membranes, Journal of Experimental Botany, volume 62, no. 7, pages 2393-2402, DOI:10.1093/jxb/err034. [PDF]
• Sommer MS, Daum B, Gross LE, Weis BLM, Mirus O, Abram L, Maier U-G, Kühlbrandt W, Schleiff E. (2011) Chloroplast Omp85 proteins change orientation during evolution, Proceedings of the National Academy of Sciences, volume 108, no. 33, pages 13841-13846, DOI:10.1073/pnas.1108626108. [PDF]
• Davies KM, Strauss M, Daum B, Kief JH, Osiewacz HD, Rycovska A, Zickermann V, Kühlbrandt W. (2011) Macromolecular organization of ATP synthase and complex I in whole mitochondria, Proceedings of the National Academy of Sciences, volume 108, no. 34, pages 14121-14126, DOI:10.1073/pnas.1103621108. [PDF]

2010

• Daum B, Nicastro D, Austin J, McIntosh JR, Kühlbrandt W. (2010) Arrangement of Photosystem II and ATP Synthase in Chloroplast Membranes of Spinach and Pea  , The Plant Cell, volume 22, no. 4, pages 1299-1312, DOI:10.1105/tpc.109.071431. [PDF]
• Brust D, Daum B, Breunig C, Hamann A, Kühlbrandt W, Osiewacz HD. (2010) Cyclophilin D links programmed cell death and organismal aging in Podospora anserina, Aging Cell, volume 9, no. 5, pages 761-775, DOI:10.1111/j.1474-9726.2010.00609.x. [PDF]

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