Photo of Dr Bertram Daum

Dr Bertram Daum

Research Fellow

Email:

Telephone: 01392 727455

Extension: (Streatham) 7455

Dr Bertram Daum

Research Fellow at the Living Systems Institute

College of Mathematics, Engineering and Physical Science

Telephone: 01392727455

Room: T03.17, Living Systems Institute

 

Follow me on Twitter: @DaumLaboratory

 

Research

Archaea are ubiquitous microorganisms that are the ancestors of all eukaryotic life and inhabit diverse environments ranging from the most extreme to the human body. Archaea that are part of the human microbiome are increasingly recognised as key health factors in metabolic conditions such as obesity. Moreover, protein complexes derived from extremophilic archaea are ultra-stable and highly resilient to extreme conditions such as heat, pH and salt and are thus of great interest for bio- and nanotechnology. My lab employs state-of-the-art single particle electron cryo-microscopy and cryo-tomography to investigate the structure and function of archaeal surface proteins. These include S-layers, which form highly stable cage-like proteinaceous cell walls and the archaellum, a filamentous molecular machine used for rotary propulsion, surface adherence, biofilm formation and cell-cell communication. Our work will provide detailed information about how archaea move through and interact with their (microbiomal) environment and inform new approaches to exploit s-layers and the archaellum machinery in nanotechnology and drug delivery.

 

In the news: 

http://www.exeter.ac.uk/news/featurednews/title_603910_en.html

https://phys.org/news/2017-08-scientists-snapshots-molecular-propeller-degrees.html

http://www.sciencenewsline.com/news/2017082417090076.html

http://www.hitechdays.com/browser/253317/

 

Equipment

We are currently setting up our wetlab including cultivation equipment for thermophiles, a cutting-edge GPU computer cluster for image processing, a cryo-capable FEI Tecnai Spirit electron microscope for sample screening, as well as a brand-new GW4-shared 200 kV FEI Talos Arctica cryo electron microscope for high resolution imaging.

 

Qualifications

2014: Dr phil. nat. in Biology, University of Frankfurt, Germany

2008:  MsSc in Biology, University of Kassel, Germany

 

Career

2017 – present: Research Fellow at the Living Systems Institute, University of Exeter, UK

2014 – 2017: Postdoctoral researcher at the Max-Planck-Institute of Biophysics, Frankfurt, Germany   

2008 – 2014: PhD research at the Max-Planck-Institute of Biophysics, Frankfurt, Germany   

 

Awards

2014: Otto-Hahn Medal of the Max Planck Society

2014: Young Investigator’s Award of the Bethge Foundation for Electron Microscopy

 

Publications

Daum B#, Vonck J, Bellack A, Chaudhury P, Reichelt R, Albers SV, Rachel R, Kühlbrandt W (2017) Structure and in situ organisation of the Pyrococcus furiosus archaellum machinery. Elife, Jun 27;6. pii: e27470. doi: 10.7554/eLife.27470.

Daum B, Auerswald A, Gruber T, Hause G, Balbach J, Kühlbrandt W†, Meister A (2016)  Supramolecular organization of the human N-BAR domain and its role in shaping the sarcolemma membrane. Journal of Structural Biology, 194(3):375-82.

Perras A, Daum B, Ziegler C, Takahashi LK, Ahmed M, Wanner G, Klingl G, Leitinger G, Kolb-Lenz D, Gribaldo S, Auerbach A, Mora M, Probst AJ, Bellack A and Moissl-Eichinger C (2015) S-layers at a second glance? Altiarchaeal grappling hooks (hami) resemble archaeal S-layer proteins in structure and sequence. Frontiers in Microbiology 6:543

Davies KM, Daum B, Gold V, Mühleip AW, Brandt T, Blum TB, Mills DJ, Kühlbrandt W (2014) Visualization of  ATP-synthase dimers in mitochondria by electron cryotomography. Journal of Visualized Experiments, (91):51228

Daum B*, Quax TEF*, Sachse M, Mills D, Reimann J, Yildiz Ö, Häder S, Saveanu C, Forterre P, Albers SV, Kühlbrandt W and Prangishvili D† (2014) Self-assembly of the universal membrane-remodeling protein PVAP into 7-fold virus-associated pyramids, Proceedings of the National Academy of Sciences of the United States of America, 111(10):3829-34

Quemin ERJ, Lucas S, Daum B, Quax EFT, Kühlbrandt W, Forterre P, Albers SV, Prangishvili D, Krupovic M. (2013) First insights into the entry process of hyperthermophilic archaeal viruses, Journal of Virology 87(24):13379-85.

Daum B, Walter A, Horst A, Osiewacz H, Kühlbrandt W (2013) Age-dependent dissociation of ATP synthase dimers and loss of inner membrane cristae in mitochondria. Proceedings of the National Academy of Sciences of the United States of America 111:15301-10

Davies KM & Daum B (2013) Role of cryo-ET in membrane bioenergetics research. Biochemical Society Transactions 41(5):1227-34.

Sommer MS*, Daum B*, Gross LE, Weis BL, Mirus O, Abram L, Maier UG, Kühlbrandt W, Schleiff E (2011) Chloroplast Omp85 proteins change orientation during evolution. Proceedings of the National Academy of Sciences of the United States of America 108: 13841-13846

Davies KM*, Strauss M*, Daum B*, Kief JH, Osiewacz HD, Rycovska A, Zickermann V, Kühlbrandt W (2011) Macromolecular organization of ATP synthase and complex I in whole mitochondria. Proceedings of the National Academy of Sciences of the United States of America 108: 14121-14126

Daum B & Kühlbrandt W (2011) Electron tomography of plant thylakoid membranes. Journal of Experimental Botany 62: 2393-2402

Brust D, Daum B, Breunig C, Hamann A, Kühlbrandt W, Osiewacz HD (2010) Cyclophilin D links programmed cell death and organismal aging in Podospora anserina. Aging Cell 9:      761-775

Daum B, Nicastro D, Austin J, 2nd, McIntosh JR, Kühlbrandt W (2010) Arrangement of photosystem II and ATP synthase in chloroplast membranes of spinach and pea. The Plant Cell 22: 1299-1312.

#corresponding author

* shared 1st Author

 

 

 

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