Fluorescence microscopy image from a DPPC monolayer doped with NBD-PC. The domain size is approximately 35 µm.
One of our Langmuir troughs mounted in Station 16.2 at the Daresbury Synchrotron Radiation Source.
Geometry used in GIXD.Langmuir monolayers
Current studies include:
- Structure and properties of the pre-ocular tear film
- Lipid monolayers from normal and abnormal cell membranes
- Interactions between skeletal proteins and lipid monolayers
Pressure-Area Isotherms and Surface Electrostatic Potential
These classical techniques give information about the overall phase behaviour and the electrostatic properties of the monomolecular layer spread on the liquid surface.
Pressure-area isotherm of a DMPC monolayer.Fluorescence Microscopy from Langmuir Monolayers
Fluorescence Microscopy can be used to visualise domain structures in lipid monomolecular layers formed on the water/air interface at different surface pressures, and to investigate the effects of various solutes such as ions and proteins in the aqueous subphase on domain structure. The domain structure of membrane lipids is believed to be important to the function. We use fluorescence microscopy to detect lipid domain formation and dynamics.
Grazing Incidence X-Ray Diffraction (GIXD) from Langmuir Monolayers
The 2D order in the monolayer can be resolved using GIXD. The methods gives valuable information about change in molecular ordering due to interactions with solutes such as skeletal proteins or changes in the surface pressure.
Example of an X-ray diffraction pattern from 16:0 PS.
