MIRA 900D
MIRA OPO
Levante OPO
Adjacent Cell Culture Room
Adjacent wet lab facility

Multiphoton imaging and spectroscopy laboratory

Equipment

The front-end of our system is a modified Olympus IX71 inverted microscope. Dual sources, a pico-second OPO tunable from 700 – 1400nm (Levante Emerald from APE, Berlin), and a switchable femto- and pico-second system tunable from 700 – 1600nm (MIRA-D, and MIRA OPO from Coherent, USA) provide the multiple contrast modalities; Multiphoton fluorescence, Second- and Third-Harmonic Generation (SHG & THG) and Coherent Anti-stokes Raman Scattering (CARS). 

MIRA 900D

  • Dual femto- and pico- second operation
  • In femto mode provides excitation for second harmonic generation, multiphoton fluorescence, multiplex CARS and time-resolved CARS
  • In pico mode provides excitation for frequency modulated CARS
  • Output power: 1.2 W
  • Wavelength Range: 690 - 990 nm
  • Pulse width (femto mode): ~100 fs
  • Pulse width (pico mode): ~1 ps

MIRA OPO

  • Dual femto- and pico- second operation
  • In femto mode provides excitation for third harmonic generation, sum frequency generation, pump-probe microscopy
  • In pico mode provides excitation for frequency modulated CARS
  • Output power: 300 mW
  • Wavelength Range: 1050 - 1600 nm
  • Pulse width (femto mode): ~120 fs
  • Pulse width (pico mode): ~2 ps

Levante OPO

  • This is a picosecond OPO pumped by a frequency doubled Nd:YVO-Laser (523 nm). The signal and idler are tuneable from 690 to 990 nm and 1150 to 2300 nm respectively.
  • There is perfect spatial and temporal overlap of signal and idler beam, which can be outcoupled on one joint beam axis.

Multiphoton Imaging

  • A modified Olympus FV300 and IX71 allows multiphoton imaging.

Adjacent Cell Culture Room

  • Class II cell culture hood.
  • Incubator.

Adjacent wet lab facility

Multiphoton Imaging equipment
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