Multiphoton imaging and spectroscopy laboratory

Equipment

The front-end of our system is a modified Olympus IX71 inverted microscope. Dual sources, a pico-second OPO tunable from 700 – 1400nm (Levante Emerald from APE, Berlin), and a switchable femto- and pico-second system tunable from 700 – 1600nm (MIRA-D, and MIRA OPO from Coherent, USA) provide the multiple contrast modalities; Multiphoton fluorescence, Second- and Third-Harmonic Generation (SHG & THG) and Coherent Anti-stokes Raman Scattering (CARS). 

MIRA 900D

• Dual femto- and pico- second operation
• In femto mode provides excitation for second harmonic generation, multiphoton fluorescence, multiplex CARS and time-resolved CARS
• In pico mode provides excitation for frequency modulated CARS
• Output power: 1.2 W
• Wavelength Range: 690 - 990 nm
• Pulse width (femto mode): ~100 fs
• Pulse width (pico mode): ~1 ps
  

MIRA OPO

• Dual femto- and pico- second operation
• In femto mode provides excitation for third harmonic generation, sum frequency generation, pump-probe microscopy
• In pico mode provides excitation for frequency modulated CARS
• Output power: 300 mW
• Wavelength Range: 1050 - 1600 nm
• Pulse width (femto mode): ~120 fs
• Pulse width (pico mode): ~2 ps

Levante OPO

• This is a picosecond OPO pumped by a frequency doubled Nd:YVO-Laser (523 nm). The signal and idler are tuneable from 690 to 990 nm and 1150 to 2300 nm respectively.
• There is perfect spatial and temporal overlap of signal and idler beam, which can be outcoupled on one joint beam axis.

Multiphoton Imaging

• A modified Olympus FV300 and IX71 allows multiphoton imaging.

Adjacent Cell Culture Room

• Class II cell culture hood.
• Incubator.

Adjacent wet lab facility