Friday 16 Dec 2016: Light microscopy with electrons, electron microscopy with light
Dr Jacob Hoogenboom - Delft
Newmann Red 12:30-12:30
Since the early work of Antoni van Leeuwenhoek, developments in light microscopy (LM) have been instrumental for progress in many areas of science. In recent years, both light and electron microscopy (EM) have seen revolutionary developments with typical spatial scales almost overlapping. Correlation microscopy has emerged to show the distribution of molecules visible with LM within a high-resolution image of the structural environment obtained with EM. Integrated light-electron microscopes have led to easier procedures for obtaining correlated images. Here, I will show that besides very precise correlation, the integrated approach can also lead to new imaging techniques crossing the traditional boundaries between both types of microscopy. In our system, an electron beam can be positioned anywhere within the field of view of a fluorescence microscope. In this way, we can do light microscopy using electrons, and electron microscopy guided by light. I will provide application examples ranging from biology to nanophotonics: electron-beam induced fluorescence superresolution, ‘snapshot’-electron microscopy of live cells, multi-color electron microscopy, and time-resolved electron microscopy probing active medium inside metal-coated nanolasers.
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 A.C. Zonnevylle et al., Journal of Microscopy 252, 58-70 (2013).
 N. Liv et al, ACS Nano 10, 265-273 (2016)